Known as the "Cleaner", Mako Yakumaru is a member of the Anti-Schoolers first sent to assassinate Kusabi Mido after he fails to kill Tsukune. She later disguises herself as the school nurse at the school's hospital, and in order to sow dissesion among Tsukune and his friends, Mako controlled and manipulated Moka's body after injecting her with a hypnotic fluid, forcing her to try to kill him. When this tactic fails, Mako injects her fluid into a multitude of bystanders, all forced to attack Tsukune. She was later defeated by Inner Moka just when her Outer half attempted to kill Tsukune after being injected a second time.
Mako appears later in an episode of Rosario + Vampire Capu2, designated as the actual school nurse rather than in disguise as one. In this adaptation, Mako helps a distraught Yukari fulfill her wish of growing up by injecting her in a similar fashion. However, this was done so she can feed on Yukari's negative emotions, and later puts her to sleep again to do so. However, she was stopped by Tsukune and company upon her discovery, with the finishing blow dealt by Inner Moka.